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Thermoluminescence measurement system
Matějka, Tomáš ; Král, Jan (referee) ; Kubíček, Michal (advisor)
This thesis deals with revision and design of electronic for measuring system PSI TL 500 which is designed for measurement of thermoluminescence phenomena in photosystem II. Thesis describes principles and properties of the measuring system in its current form and changes for the new version are discussed. Digital control part based on Xilinx Zynq platform is designed. Revision of analog measuring part of system is made and partial changes are implemented.
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Thermoluminescence measurement system
Matějka, Tomáš ; Král, Jan (referee) ; Kubíček, Michal (advisor)
This thesis deals with revision and design of electronic for measuring system PSI TL 500 which is designed for measurement of thermoluminescence phenomena in photosystem II. Thesis describes principles and properties of the measuring system in its current form and changes for the new version are discussed. Digital control part based on Xilinx Zynq platform is designed. Revision of analog measuring part of system is made and partial changes are implemented.
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The thermostability of photosystem II photochemistry is related to maintenance of thylakoid membranes organization
Karlický, Václav ; Kurasová, I. ; Špunda, Vladimír
For higher plant photosynthetic reactions, responses to the temperature changes are important, particularly if we consider global warming and the increasing frequency of extreme temperature fluctuations. High temperature stress decreases photosynthetic assimilation through the inactivation of photosystem II (PSII), the most heat-sensitive component of the oxygen-evolving complex. We have recently found higher thermostability of spruce PSII photochemistry compared to such control plants as Arabidopsis species and barley. In this work, we have therefore attempted to describe the causes of this effect on the level of the organization of pigment–protein complexes (PPCs) in spruce thylakoid membranes using circular dichroism (CD) spectroscopy. We have confirmed higher maximum efficiency of PSII photochemistry (FV/FM) for spruce needles in comparison to barley leaves. Temperature-dependent CD spectra have also demonstrated higher (by about 6°C) PSII thermostability of chiral macro-organization of PPCs in spruce thylakoid membranes compared to those in barley. However, thermal disruption of PPCs did not reveal significant differences. Our results demonstrate that the stability of PSII macro-organization in different plant species correlates with the thermostability of PSII photochemistry in intact needles/leaves and so effective PSII photochemistry is related to the maintenance of PSII macro-organization under high temperature stress.
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Mobility of photosynthetic proteins
KRAFL, Jaroslav
Mobility of pigment-protein complexes (phycobilizomes and photosystem II playing a key role in photosynthesis) was studied by FRAP method (Fluorescence Recovery After Photobleaching). FRAP represents a fluorescence based microscopy method enabling measurement of protein mobility in living systems. The protein complexes are bleached by a laser pulse. And mobility of unbleached proteins is measured as a fluorescence recovery in the bleached area. Currently we have only limited knowledge about the mobility of photosynthetic proteins. This work was aimed at optimization of the photosynthetic protein mobility measurement by FRAP. I have performed several methodological experiments which led to the successful assessment of phycobilisome and chlorophyll-containing proteins diffusion coefficients in selected red algae (Porfyridium cruentum, Cyanidium caldarium) and cyanobacteria (Synechocystis PCC6803, Acaryochloris marina). The methodology developed and validated in my thesis was then applied in further research projects.
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